NestLink

This is the development version of NestLink; for the stable release version, see NestLink.

NestLink an R data package to guide through Engineered Peptide Barcodes for In-Depth Analyzes of Binding Protein Ensembles


Bioconductor version: Development (3.20)

Provides next-generation sequencing (NGS) and mass spectrometry (MS) sample data, code snippets and replication material used for developing NestLink. The NestLink approach is a protein binder selection and identification technology able to biophysically characterize thousands of library members at once without handling individual clones at any stage of the process. Data were acquired on NGS and MS platforms at the Functional Genomics Center Zurich.

Author: Pascal Egloff [aut] , Iwan Zimmermann [ctb] , Fabian M. Arnold [ctb], Cedric A.J. Hutter [ctb] , Lennart Opitz [aut, cre] , Lucy Poveda [ctb] , Hans-Anton Keserue [ctb], Christian Panse [aut, ctb] , Bernd Roschitzki [aut] , Markus Seeger [aut]

Maintainer: Lennart Opitz <lopitz at fgcz.ethz.ch>

Citation (from within R, enter citation("NestLink")):

Installation

To install this package, start R (version "4.4") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

# The following initializes usage of Bioc devel
BiocManager::install(version='devel')

BiocManager::install("NestLink")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("NestLink")
0. Simulate flycode properties. HTML R Script
1. Link high quality flycode and nanobody sequences by NGS filtering. HTML R Script
2. Analyze flycode detectability using ESP and SSRC prediction HTML R Script
3. Compare Predicted and Observed flycodes hydrophobicity values using F255744. HTML R Script
4. Control experiment to assess the robustness of protein detection via flycodes (NMETH-A35040 Suppl. notes 1). HTML R Script
5. make-data HTML R Script
Reference Manual PDF
NEWS Text

Details

biocViews ExperimentData, ExperimentHub, MassSpectrometryData, ReproducibleResearch, SequencingData
Version 1.21.0
License GPL
Depends R (>= 3.6), AnnotationHub(>= 2.15), ExperimentHub(>= 1.0), Biostrings(>= 2.51), gplots (>= 3.0), protViz (>= 0.4), ShortRead(>= 1.41)
Imports grDevices, graphics, stats, utils
System Requirements
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Suggests BiocStyle(>= 2.2), DT, ggplot2, knitr, rmarkdown, testthat, specL, lattice, scales
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package NestLink_1.21.0.tar.gz
Windows Binary
macOS Binary (x86_64)
macOS Binary (arm64)
Source Repository git clone https://git.bioconductor.org/packages/NestLink
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/NestLink
Package Short Url https://bioconductor.org/packages/NestLink/
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