SpotSweeper

Spatially-aware quality control for spatial transcriptomics


Bioconductor version: Release (3.20)

Spatially-aware quality control (QC) software for both spot-level and artifact-level QC in spot-based spatial transcripomics, such as 10x Visium. These methods calculate local (nearest-neighbors) mean and variance of standard QC metrics (library size, unique genes, and mitochondrial percentage) to identify outliers spot and large technical artifacts. Scales linearly with the number of spots and is designed to be used with 'SpatialExperiment' objects.

Author: Michael Totty [aut, cre] , Boyi Guo [aut]

Maintainer: Michael Totty <mictott at gmail.com>

Citation (from within R, enter citation("SpotSweeper")):

Installation

To install this package, start R (version "4.4") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("SpotSweeper")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

Reference Manual PDF
NEWS Text
LICENSE Text

Details

biocViews GeneExpression, QualityControl, Software, Spatial, Transcriptomics
Version 1.1.0
In Bioconductor since BioC 3.19 (R-4.4) (0.5 years)
License MIT + file LICENSE
Depends R (>= 4.3.0)
Imports SpatialExperiment, SummarizedExperiment, BiocNeighbors, SingleCellExperiment, stats, escheR, MASS, ggplot2, spatialEco, grDevices
System Requirements
URL https://github.com/MicTott/SpotSweeper
Bug Reports https://support.bioconductor.org/tag/SpotSweeper
See More
Suggests knitr, BiocStyle, rmarkdown, scuttle, STexampleData, ggpubr, testthat (>= 3.0.0)
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Package Archives

Follow Installation instructions to use this package in your R session.

Source Package
Windows Binary (x86_64)
macOS Binary (x86_64)
macOS Binary (arm64) SpotSweeper_1.1.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/SpotSweeper
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/SpotSweeper
Bioc Package Browser https://code.bioconductor.org/browse/SpotSweeper/
Package Short Url https://bioconductor.org/packages/SpotSweeper/
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